The AGEhIV cohort study is an ongoing, prospective, cohort study in Amsterdam. A total of 598 HIV-1-infected participants and 550 HIV-uninfected controls, with an age ≥ 45 years, from the same geographic region and with similar socio-demographic and behavioral risk factors were enrolled between 2010 and 2012. This paper reports the cross sectional analysis of age-associated non communicable comorbidities (AANCCs) prevalence, including cardiovascular, metabolic, pulmonary, renal, bone, and malignant disease, as well as risk factors in this cohort. HIV-infected participants (n=540) had a significantly higher mean number of AANCCs than controls (n=524): 1.3 (SD, 1.14) versus 1.0 (SD, 0.95), p < 0.001. Significantly more HIV-infected participants had ≥ 1 AANCC (69.4% versus 61.8%, p=0.009). Hypertension, myocardial infarction, peripheral arterial disease, and impaired renal function were significantly more prevalent among HIV-infected participants. Risk of AANCC by ordinal logistic regression was independently associated with age, smoking, positive family history for cardiovascular/metabolic disease, and higher waist-to-hip ratio, but also with HIV infection (odds ratio, 1.58 [95% confidence interval, 1.23-2.03]; p < .001). In those with HIV, longer exposure to CD4 counts < 200 cells/μL, and, to a lesser extent, higher levels of high-sensitivity C-reactive protein and soluble CD14, and longer prior use of high-dose ritonavir (≥400 mg/24 hours) were each also associated with a higher risk of AANCCs.